Mexican Culture Ampoule

Application of the Mexican Culture Ampoule

1. Disinfect and unpack

First of all, wash your hands thoroughly and dab the outside of the Mexian Culture ampoule with a cloth or swab soaked in alcohol. Then you examine the packaging of the syringe for damage. If undamaged, the syringe is still sterile. Open the packaging.

2. Fill syringe

The ampoule with the culture liquid has a rubber seal, the so-called septum. This can be punctured with the syringe, but prevents air and contaminants from entering the bottle. Shake the bottle vigorously once, then remove the protective cap from the septum. The rubber seal is first disinfected with some alcohol and then pierced with the syringe. Now you can pull up the solution. If you have problems opening the syringe because of the resulting vacuum, you can easily loosen the cap of the ampoule at one point. Under certain circumstances, however, this can lead to the solution becoming contaminated with impurities.

3. Inoculation of the substrate

Then you can start inoculating. To do this, carefully drip the solution onto your substrate using the syringe. Be sure to spread the drops evenly, as each drop becomes a potential fungal growth spot. You only need 3 milliliters of solution for 1 liter of substrate.

retention and reuse

If there is some leftover from the Mexican Culture Ampoule, you can put the protective cap back on the septum and store it in the fridge at 2 to 4 degrees. It can be kept for up to 2 years.